/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-TLE1 Antibody
Primary Antibodies  

Rabbit anti-TLE1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

TLE1

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

transducin-like enhancer protein 1 (TLE1) is a transcriptional corepressor that binds to a number of transcription factors. TLE1 Inhibits NF-kappa-B-regulated gene expression and inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q04724].

7088

TLE1

transducin like enhancer of split 1

Q04724

Transducin-like enhancer protein 1

Alternate Names

E(Sp1) homolog; enhancer of split groucho-like protein 1; ESG; ESG1; GRG1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin-like enhancer protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.