Bethyl Laboratories
Rabbit anti-TMED10/TMP21 Antibody Affinity Purified
Primary
Catalog #A305-218A
TargetTMED10/TMP21
HostRabbit
ClonalityPolyclonal
ApplicationWB
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Product Description
Transmembrane emp24 domain-containing protein 10 (TMED10) is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity [taken from NCBI Entrez Gene (Gene ID: 10972)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetTMED10/TMP21
Antigen Species
Human
Gene ID10972
Gene Symbol
TMED10
Gene Name
transmembrane p24 trafficking protein 10
Uniprot IDP49755
Protein Name
Transmembrane emp24 domain-containing protein 10
Alternate Names
21 kDa transmembrane trafficking protein • 21 kDa transmembrane-trafficking protein • p23 • p24 family protein delta-1 • P24(DELTA) • p24d1 • p24delta • p24delta1 • S31I125 • S31III125 • testicular tissue protein Li 206 • TMP21 • Tmp-21-I • transmembrane emp24 domain-containing protein 10 • transmembrane emp24-like trafficking protein 10 • transmembrane protein Tmp21
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TMED10/TMP21 immobilized on solid support.
The epitope recognized by A305-218A maps to a region between residue 25 to 75 of human Transmembrane emp24 domain-containing protein 10 using the numbering given in entry P49755.2 (GeneID 10972).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-218A · Lot 3 (current lot)Open
Product DatasheetPDF · A305-218A · Lot 3 (current lot)Open
Safety Data SheetPDF · A305-218AOpen
Services
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