Rabbit anti-TMTC3 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to TMTC3 immobilized on solid support.

The epitope recognized by A305-776A maps to a region between residue 825 and 875 of human Transmembrane and TPR repeat-containing protein 3 using the numbering given in entry Q6ZXV5.2 (GeneID 160418).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.