Bethyl Laboratories
Rabbit anti-TOM1 Antibody Affinity Purified
Primary
Catalog #A301-362A
TargetTOM1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
TOM1 (target of myb1) was originally identified as a gene activated by the expression of oncogenic Myb1. TOM1 has been shown to form a complex with tollip (toll-interacting protein) and to bind ubiquitin chains. It has also been shown to bind to clathrin heavy chain and is proposed to function to link polyubiquitinated proteins to clathrin and regulate endosomal trafficking of ubiquitinated proteins. Recent evidence shows that TOM1 bridges endofin to clathrin and is critical for the endosomal targeting of clathrin by endofin.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 442 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTOM1
Antigen Species
Human
Gene ID10043
Gene Symbol
TOM1
Gene Name
target of myb1 membrane trafficking protein
Uniprot IDO60784
Protein Name
Target of Myb protein 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TOM1 immobilized on solid support.
The epitope recognized by A301-362A maps to a region between residue 450 and 492 of human target of myb1 using the numbering given in entry NP_005479.1 (GeneID 10043).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-362A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-362A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-362AOpen
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