Bethyl Laboratories
Rabbit anti-Topo II Beta Antibody Affinity Purified
Primary
Catalog #A300-950A
TargetTopo II Beta
HostRabbit
ClonalityPolyclonal
ApplicationIHC, IP, WB
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Product Description
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II beta is one of two described isoforms that are likely products of a gene duplication event. TopoII beta is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1576 and C-terminus
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTopo II Beta
Antigen Species
Human
Gene ID7155
Gene Symbol
TOP2B
Gene Name
topoisomerase (DNA) II beta
Uniprot IDQ02880
Protein Name
DNA topoisomerase 2-beta
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Topo II Beta immobilized on solid support.
The epitope recognized by A300-950A maps to a region between residue 1570 and the C-terminus (residue 1621) of human DNA Topoisomerase II Beta using the numbering given in entry NP_001059.2 (GeneID 7155).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-950A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-950A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-950AOpen
Citations / Publications
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