Bethyl Laboratories
Rabbit anti-TOPORS IHC Antibody Affinity Purified
Primary
Catalog #IHC-00629
TargetTOPORS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
TOPORS (topoisomerase I-binding RS protein) was identified in a a yeast two-hybrid screen for proteins that bind the N-terminus of topoisomerase I. TOPORS contains a RING-type zinc-finger domain and an RS-rich region. TOPORS has been found to possess both ubiquitin and SUMO-1 E3 ligase activity. It can ubiquitinate and sumoylate p53 and may possibly function as a tumor suppressor. Defects in TOPORS are the cause of retinitis pigmentosa type 31.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 995 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetTOPORS
Antigen Species
Human
Gene ID10210
Gene Symbol
TOPORS
Gene Name
TOP1 binding arginine/serine rich protein
Uniprot IDQ9NS56
Protein Name
E3 ubiquitin-protein ligase Topors
Alternate Names
E3 ubiquitin-protein ligase Topors • LUN • p53-binding protein 3 • P53BP3 • RING-type E3 ubiquitin transferase Topors • RP31 • SUMO1-protein E3 ligase Topors • topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase • Topoisomerase I-binding arginine/serine-rich protein • topoisomerase I-binding RING finger protein • TP53BPL • tumor suppressor p53-binding protein 3
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TOPORS immobilized on solid support.
The epitope recognized by IHC-00629 maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00629Open
Product DatasheetPDF · IHC-00629 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00629 · Lot 1 (current lot)Open
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