Bethyl Laboratories
Rabbit anti-TR2 Antibody Affinity Purified
Primary
Catalog #A303-047A
TargetTR2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
TR2 is an orphan nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes [taken from NCBI Entrez Gene (Gene ID: 7181].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 553 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetTR2
Antigen Species
Human
Gene ID7181
Gene Symbol
NR2C1
Gene Name
nuclear receptor subfamily 2 group C member 1
Uniprot IDP13056
Protein Name
Nuclear receptor subfamily 2 group C member 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TR2 immobilized on solid support.
The epitope recognized by A303-047A maps to a region between residue 553 and 603 of human Testicular Receptor 2 using the numbering given in entry NP_003288.2 (GeneID 7181).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-047A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-047A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-047AOpen
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