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Rabbit anti-TRAF6 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
TRAF6
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 472 and 522
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
100 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to TRAF6 immobilized on solid support.

The epitope recognized by A303-803A-T maps to a region between residue 472 and 522 of human TNF Receptor-Associated Factor 6 using the numbering given in entry NP_004611.1 (GeneID 7189).

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Gene & Protein Information

TNF receptor-associated factor 6 (TRAF6) is an E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. TRAF6 also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. TRAF6 leads to the activation of NF-kappa-B and JUN. TRAF6 may be essential for the formation of functional osteoclasts, and it regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. TRAF6 also seems to play a role in dendritic cells (DCs) maturation and/or activation. TRAF6 represses c-Myb-mediated transactivation, in B-lymphocytes. TRAF6 is an adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y4K3].

7189

TRAF6

TNF receptor associated factor 6

Q9Y4K3

TNF receptor-associated factor 6

Alternate Names

E3 ubiquitin-protein ligase TRAF6, interleukin-1 signal transducer, MGC:3310, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6, RNF85, TNF receptor-associated factor 6, TNF receptor-associated factor 6, E3 ubiquitin protein ligase

Applications

Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.