/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-TRAP100/MED24 Antibody
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Primary Antibodies  

Rabbit anti-TRAP100/MED24 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (7)
Target:

TRAP100/MED24

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 939 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Chicken,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to TRAP100/MED24 immobilized on solid support.

The epitope recognized by A301-472A maps to a region between residue 939 and 989 of human thyroid hormone receptor-associated protein complex 100 kDa component (mediator complex subunit 24 ) using the numbering given in entry NP_055630.2 (GeneID 9862).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

TRAP100/MED24 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. TRAP100/MED24 associates with mediator subunit TRAP220. Both are essential to mouse development and play a role in androgen receptor-mediated transcription. Studies in zebrafish have shown that TRAP100/MED24 is also important to retinal development.

9862

MED24

mediator complex subunit 24

O75448

Mediator of RNA polymerase II transcription subunit 24

Alternate Names

activator-recruited cofactor 100 kDa component; ARC100; cofactor required for Sp1 transcriptional activation subunit 4; cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa; CRSP complex subunit 4; CRSP100; CRSP4; DRIP100; hTRAP100; MED5; Mediator complex subunit 24; mediator of RNA polymerase II transcription subunit 24; mediator of RNA polymerase II transcription, subunit 24 homolog; THRAP4; thyroid hormone receptor-associated protein 4; thyroid hormone receptor-associated protein complex 100 kDa component; TRAP100; vitamin D3 receptor-interacting protein complex 100 kDa component; vitamin D3 receptor-interacting protein complex component DRIP100

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.