Bethyl Laboratories
Rabbit anti-TRIM25 Antibody Affinity Purified
Primary
Catalog #A301-856A
TargetTRIM25
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
TRIM (tripartite motif) proteins, also known as RBCC (RING, B-box, coiled-coil) proteins, are defined by the presence of a tripartite structural motif composed of a RING domain, one or two B-box motifs, and a coiled-coil region. TRIM proteins have been found to be involved in a variety of cellular processes such as apoptosis, proliferation, differentiation, and innate immunity. Of these processes, the most intriguing is their involvement in the innate immune response. TRIM25 has been demonstrated to mediate ubiquitination of the cytosolic receptor retinoic acid-inducible gene-I (RIG-I) in response to viral infection.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTRIM25
Antigen Species
Human
Gene ID7706
Gene Symbol
TRIM25
Gene Name
tripartite motif containing 25
Uniprot IDQ14258
Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
Alternate Names
E3 ubiquitin/ISG15 ligase TRIM25 • EFP • estrogen-responsive finger protein • RING finger protein 147 • RING-type E3 ubiquitin transferase • RING-type E3 ubiquitin transferase TRIM25 • RNF147 • tripartite motif protein TRIM25 • tripartite motif-containing protein 25 • ubiquitin/ISG15-conjugating enzyme TRIM25 • Z147 • Zinc finger protein 147 • zinc finger protein 147 (estrogen-responsive finger protein) • zinc finger protein-147 • ZNF147
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TRIM25 immobilized on solid support.
The epitope recognized by A301-856A maps to a region between residue 75 and 125 of human tripartite motif-containing 25 using the numbering given in entry NP_005073.2 (GeneID 7706).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-856A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-856A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-856AOpen
Citations / Publications
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