Bethyl Laboratories
Rabbit anti-TRIM33/TIF1gamma IHC Antibody Affinity Purified
Primary
Catalog #IHC-00216
TargetTRIM33/TIF1gamma
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
TRIM33 is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM33 functions as an E3 ubiquitin ligase and plays a role in the TGFbeta/SMAD pathway.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1077 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetTRIM33/TIF1gamma
Antigen Species
Human
Gene ID51592
Gene Symbol
TRIM33
Gene Name
tripartite motif containing 33
Uniprot IDQ9UPN9
Protein Name
E3 ubiquitin-protein ligase TRIM33
Alternate Names
E3 ubiquitin-protein ligase TRIM33 • ECTO • ectodermin homolog • protein Rfg7 • PTC7 • RET-fused gene 7 protein • RFG7 • RING-type E3 ubiquitin transferase TRIM33 • TF1G • TIF1G • TIF1GAMMA • TIF1-gamma • TIFGAMMA • Transcription intermediary factor 1-gamma • transcriptional intermediary factor 1 gamma • Tripartite motif-containing protein 33
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TRIM33/TIF1gamma immobilized on solid support.
The epitope recognized by IHC-00216 maps to a region between residue 1077 and the C-terminus (residue 1127) of human tripartite motif-containing 33 (transcriptional intermediary factor 1 gamma) using the numbering given in entry NP_056990.3 (GeneID 51592).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00216Open
Product DatasheetPDF · IHC-00216 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00216 · Lot 1 (current lot)Open
Citations / Publications
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