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Rabbit anti-TRIP4/ASC1-1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

TRIP4/ASC-1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 75 and 125

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to TRIP4/ASC-1 immobilized on solid support.

The epitope recognized by A300-203A maps to a region between residues 75 and 125 of human thyroid hormone receptor interactor 4, (activating signal cointegrator 1) using the number given in entry NP_057297.2 (GeneID 9325).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

Thyroid receptor interacting protein 4 (TRIP4) is also known as activating signal cointegrator 1 (ASC-1). TRIP4/ASC-1 was identified as a protein that interacts with the thyroid receptor. It has been shown to act as a transcriptional co-activator of the thyroid receptor (TR), 9-cis retinoic acid (RXR), androgen receptor (AR) and estrogen.

9325

TRIP4

thyroid hormone receptor interactor 4

Q15650

Activating signal cointegrator 1

Alternate Names

activating signal cointegrator 1; ASC1; ASC-1; HsT17391; MDCDC; SMABF1; thyroid receptor-interacting protein 4; TR-interacting protein 4; TRIP-4; ZC2HC5; zinc finger, C2HC5-type

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.