Bethyl Laboratories
Rabbit anti-TRM6 Antibody Affinity Purified
Primary
Catalog #A303-008A
TargetTRM6
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6 is a substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UJA5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 447 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetTRM6
Antigen Species
Human
Gene ID51605
Gene Symbol
TRMT6
Gene Name
tRNA methyltransferase 6
Uniprot IDQ9UJA5
Protein Name
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TRM6 immobilized on solid support.
The epitope recognized by A303-008A maps to a region between residue 447 and 497 of human tRNA Methyltransferase 6 using the numbering given in entry NP_057023.2 (GeneID 51605).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-008A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-008A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-008AOpen
Citations / Publications
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