Bethyl Laboratories
Rabbit anti-U2AF35 Antibody Affinity Purified
Primary
Catalog #A302-079A
TargetU2AF35
HostRabbit
ClonalityPolyclonal
ApplicationIHC, IP, WB
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Product Description
U2AF35 heterodimerizes with U2AF65 to form the U2 snRNP auxiliary factor which is part of the splicesome. U2AF35 is required for splicing of AG-dependent pre-mRNAs and functions in recognizing the conserved 3’ splice site dinucleotide AG. U2AF35 contains an atypical RNA recognition motif (RRM), two C3H1-type zinc fingers, and a C-terminal arginine/serine (RS)-rich domain interrupted by glycines.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetU2AF35
Antigen Species
Human
Gene ID7307
Gene Symbol
U2AF1
Gene Name
U2 small nuclear RNA auxiliary factor 1
Uniprot IDQ01081
Protein Name
Splicing factor U2AF 35 kDa subunit
Alternate Names
FP793 • RN • RNU2AF1 • splicing factor U2AF 35 kDa subunit • splicing factor U2AF 35kDa subunit • U2 auxiliary factor 35 kDa subunit • U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit • U2 small nuclear RNA auxiliary factor 1 • U2 small nuclear RNA auxillary factor 1 • U2 snRNP auxiliary factor small subunit • U2(RNU2) small nuclear RNA auxiliary factor binding protein • U2AF35 • U2AFBP
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to U2AF35 immobilized on solid support.
The epitope recognized by A302-079A maps to a region between residue 140 and 190 of human U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit using the numbering given in entry NP_006749.1 (GeneID 7307).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-079A · Lot 4 (current lot)Open
Product DatasheetPDF · A302-079A · Lot 4 (current lot)Open
Safety Data SheetPDF · A302-079AOpen
Citations / Publications
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