/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-U2AF65 Antibody
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Primary Antibodies  

Rabbit anti-U2AF65 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

U2AF65

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 380 and 430

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

U2 auxiliary factor (U2AF) is comprised of a large and a small subunit and is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. Splicing factor U2 auxiliary factor 65 kDa subunit (U2AF65) is the large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. U2AF65 binds to the polypyrimidine tract of introns early during spliceosome assembly [taken from NCBI Entrez Gene (Gene ID: 11338)].

11338

U2AF2

U2 small nuclear RNA auxiliary factor 2

P26368

Splicing factor U2AF 65 kDa subunit

Alternate Names

hU2AF(65); hU2AF65; splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 snRNP auxiliary factor large subunit; U2AF65

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.