/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-UACA Antibody
NEW!
Primary Antibodies  

Rabbit anti-UACA Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

UACA

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 675 and 725

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


Request Formulation Change
Production Procedures

Antibody was affinity purified using an epitope specific to UACA immobilized on solid support.

The epitope recognized by A301-382A maps to a region between residue 675 and 725 of human uveal autoantigen with coiled-coil domains and ankyrin repeats using the numbering given in entry NP_060473.2 (GeneID 55075).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to UACA immobilized on solid support.

The epitope recognized by A301-382A-T maps to a region between residue 675 and 725 of human uveal autoantigen with coiled-coil domains and ankyrin repeats using the numbering given in entry NP_060473.2 (GeneID 55075).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves’ disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.

55075

UACA

uveal autoantigen with coiled-coil domains and ankyrin repeats

Q9BZF9

Uveal autoantigen with coiled-coil domains and ankyrin repeats

Alternate Names

nuclear membrane binding protein; NUCLING; uveal autoantigen with coiled-coil domains and ankyrin repeats

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.