/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-UACA Antibody
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Primary Antibodies  

Rabbit anti-UACA Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

UACA

Reactivity:

Human

,

Mouse

,

Rat

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1100 and 1150

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Rat,

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to UACA immobilized on solid support.

The epitope recognized by A301-383A maps to a region between residue 1075 and 1125 of human uveal autoantigen with coiled-coil domains and ankyrin repeats using the numbering given in entry NP_060473.2 (GeneID 55075).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to UACA immobilized on solid support.

The epitope recognized by A301-383A-T maps to a region between residue 1075 and 1125 of human uveal autoantigen with coiled-coil domains and ankyrin repeats using the numbering given in entry NP_060473.2 (GeneID 55075).

Additional Product Information

UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves’ disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.

55075

UACA

uveal autoantigen with coiled-coil domains and ankyrin repeats

Q9BZF9

Uveal autoantigen with coiled-coil domains and ankyrin repeats

Alternate Names

nuclear membrane binding protein; NUCLING; uveal autoantigen with coiled-coil domains and ankyrin repeats

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.