Bethyl Laboratories
Rabbit anti-UBE1 Antibody Affinity Purified
Primary
Catalog #A301-128A
TargetUBE1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
Image 1 of 3
local_shipping
Order within 19 hours and 39 minutes for shipment by Tuesday, November 17
Product Description
Ubiquitin activating enzyme 1 (UBE1) is an E1 enzyme of the three-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. UBE1 is suggested to be important for a variety of cellular processes and pathways, and its activity is regulated through phosphorylation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1008 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUBE1
Antigen Species
Human
Gene ID7317
Gene Symbol
UBA1
Gene Name
ubiquitin like modifier activating enzyme 1
Uniprot IDP22314
Protein Name
Ubiquitin-like modifier-activating enzyme 1
Alternate Names
A1S9 • A1S9T • A1S9T and BN75 temperature sensitivity complementing • A1ST • AMCX1 • CFAP124 • GXP1 • POC20 • POC20 centriolar protein homolog • Protein A1S9 • SMAX2 • testicular secretory protein Li 63 • UBA1, ubiquitin-activating enzyme E1 homolog A • UBA1A • UBE1 • UBE1X • Ubiquitin-activating enzyme E1 • ubiquitin-like modifier-activating enzyme 1
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UBE1 immobilized on solid support.
The epitope recognized by A301-128A maps to a region between residue 1008 and 1058 of human ubiquitin-activating enzyme E1 using the numbering given in entry NP_003325.2 (GeneID 7317).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-128A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-128A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-128AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1