Bethyl Laboratories
Rabbit anti-UBE4A Antibody Affinity Purified
Primary
Catalog #A304-294A
TargetUBE4A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Ubiquitin conjugation factor E4 A (UBE4A) binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14139].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUBE4A
Antigen Species
Human
Gene ID9354
Gene Symbol
UBE4A
Gene Name
ubiquitination factor E4A
Uniprot IDQ14139
Protein Name
Ubiquitin conjugation factor E4 A
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UBE4A immobilized on solid support.
The epitope recognized by A304-294A maps to a region between residue 600 to 650 of human Ubiquitination Factor E4A using the numbering given in entry NP_001191006.1 (GeneID 9354).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A304-294A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-294A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-294AOpen
Citations / Publications
Services
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