Bethyl Laboratories
Rabbit anti-UBE4B Antibody Affinity Purified
Primary
Catalog #A301-124A
TargetUBE4B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Ubiquitin conjugation factor E4 B (UBE4B) is an E4 enzyme of the multi-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. The E4 enzyme is an additional conjugation factor which is involved in polyubiquitin chain assembly. UBE4B has been identified as a candidate neuroblastoma tumor suppressor gene.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1252 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUBE4B
Antigen Species
Human
Gene ID10277
Gene Symbol
UBE4B
Gene Name
ubiquitination factor E4B
Uniprot IDO95155
Protein Name
Ubiquitin conjugation factor E4 B
Alternate Names
E4 • HDNB1 • homologous to yeast UFD2 • Homozygously deleted in neuroblastoma 1 • homozygously deleted in neuroblastoma-1 • RING-type E3 ubiquitin transferase E4 B • ubiquitin conjugation factor E4 B • Ubiquitin fusion degradation protein 2 • ubiquitination factor E4B (UFD2 homolog, yeast) • ubiquitin-fusion degradation protein 2 • UBOX3 • UFD2 • UFD2A • UFD2A-III/UBE4B-III splice isoform
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UBE4B immobilized on solid support.
The epitope recognized by A301-124A maps to a region between residue 1252 and 1302 of human ubiquitination factor E4B using the numbering given in entry AAD02233.1 (GeneID 10277).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-124A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-124A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-124AOpen
Services
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