Bethyl Laboratories
Rabbit anti-UBL7 Antibody Affinity Purified
Primary
Catalog #A302-971A
TargetUBL7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
UBL7 (ubiquitin-like protein 7) was cloned and identified from a screen of a human bone marrow stromal cell (BMSC) cDNA library. It bears a ubiquitin domain (UBQ) at its N-terminus and a ubiquitin-associated domain at its C-terminus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 330 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUBL7
Antigen Species
Human
Gene ID84993
Gene Symbol
UBL7
Gene Name
ubiquitin like 7
Uniprot IDQ96S82
Protein Name
Ubiquitin-like protein 7
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UBL7 immobilized on solid support.
The epitope recognized by A302-971A maps to a region between residue 330 and 380 of human Ubiquitin-Like 7 using the numbering given in entry NP_116296.1 (GeneID 84993).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-971A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-971A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-971AOpen
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