Bethyl Laboratories
Rabbit anti-UBR1 Antibody Affinity Purified
Primary
Catalog #A302-987A
TargetUBR1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
UBR1 is an E3 ubiquitin-protein ligase that is a component of the N-end rule pathway. UBR1 recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR1 may be involved in pancreatic homeostasis. UBR1 binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8IWV7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUBR1
Antigen Species
Human
Gene ID197131
Gene Symbol
UBR1
Gene Name
ubiquitin protein ligase E3 component n-recognin 1
Uniprot IDQ8IWV7
Protein Name
E3 ubiquitin-protein ligase UBR1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UBR1 immobilized on solid support.
The epitope recognized by A302-987A maps to a region between residue 225 and 275 of human Ubiquitin Protein Ligase E3 Component n-Recognin 1 using the numbering given in entry NP_777576.1 (GeneID 197131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-987A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-987A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-987AOpen
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