Rabbit anti-UCH-L3 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Ubiquitin carboxyl-terminal hydrolase isoenzyme 3 (UCH-L3) is a deubiquitinating enzyme (DUB) and thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P15374].
Alternate Names
testicular tissue protein Li 221; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.