Bethyl Laboratories
Rabbit anti-UCH-L3 Antibody Affinity Purified
Primary
Catalog #A302-948A
TargetUCH-L3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Ubiquitin carboxyl-terminal hydrolase isoenzyme 3 (UCH-L3) is a deubiquitinating enzyme (DUB) and thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P15374].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 180 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUCH-L3
Antigen Species
Human
Gene ID7347
Gene Symbol
UCHL3
Gene Name
ubiquitin C-terminal hydrolase L3
Uniprot IDP15374
Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UCH-L3 immobilized on solid support.
The epitope recognized by A302-948A maps to a region between residue 174 and 224 of human Ubiquitin Carboxyl-Terminal Esterase L3 using the numbering given in entry NP_005993.1 (GeneID 7347).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-948A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-948A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-948AOpen
Citations / Publications
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