Bethyl Laboratories
Rabbit anti-UCH37 Antibody Affinity Purified
Primary
Catalog #A304-099A
TargetUCH37
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Ubiquitin C-terminal hydrolase UCH37 is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. UCH37 is a deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. It is a putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y5K5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUCH37
Antigen Species
Human
Gene ID51377
Gene Symbol
UCHL5
Gene Name
ubiquitin C-terminal hydrolase L5
Uniprot IDQ9Y5K5
Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UCH37 immobilized on solid support.
The epitope recognized by A304-099A maps to a region between residue 150 to 200 of human Ubiquitin Carboxyl-Terminal Hydrolase L5 using the numbering given in entry NP_057068.1 (GeneID 51377).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-099A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-099A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-099AOpen
Citations / Publications
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