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Rabbit anti-UCH37 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
UCH37
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 175 and 225
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Pig, Bovine
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to UCH37 immobilized on solid support.

The epitope recognized by A304-100A-T maps to a region between residue 175 to 225 of human Ubiquitin Carboxyl-Terminal Hydrolase L5 using the numbering given in entry NP_057068.1 (GeneID 51377).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Ubiquitin C-terminal hydrolase UCH37 is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. UCH37 is a deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. It is a putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y5K5].

51377

UCHL5

ubiquitin C-terminal hydrolase L5

Q9Y5K5

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Alternate Names

CGI-70, INO80 complex subunit R, INO80R, ubiquitin carboxyl-terminal esterase L5, ubiquitin carboxyl-terminal hydrolase isozyme L5, ubiquitin carboxyl-terminal hydrolase L5, ubiquitin C-terminal hydrolase UCH37, ubiquitin thioesterase L5, UCH37, UCH-L5

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.