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Primary Antibodies  

Rabbit anti-UCH37 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

UCH37

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Pig,

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Ubiquitin C-terminal hydrolase UCH37 is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. UCH37 is a deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. It is a putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y5K5].

51377

UCHL5

ubiquitin C-terminal hydrolase L5

Q9Y5K5

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Alternate Names

CGI-70; INO80 complex subunit R; INO80R; ubiquitin carboxyl-terminal esterase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin thioesterase L5; UCH37; UCH-L5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.