Bethyl Laboratories
Rabbit anti-UCHL1 Recombinant Monoclonal Antibody [BLR216K]
Monoclonal Antibodies
Catalog #A700-216
TargetUCHL1
HostRabbit
CloneBLR216K
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, WB
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Product Description
Ubiquitin carboxyl-terminal hydrolase isozyme L1, or UCHL1, is a ubiquitin-protein hydrolase belonging to the peptidase C12 family. It hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 is specifically expressed in neurons and in cells of the diffuse neuroendocrine system. [taken from NCBI Entrez Gene (Gene ID: 7345)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR216K
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
12.5 µg/ml
Target Identity
TargetUCHL1
Antigen Species
Human
Gene ID7345
Gene Symbol
UCHL1
Gene Name
ubiquitin C-terminal hydrolase L1
Uniprot IDP09936
Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Alternate Names
epididymis luminal protein 117 • epididymis secretory protein Li 53 • HEL-117 • HEL-S-53 • NDGOA • neuron cytoplasmic protein 9.5 • PARK5 • PGP 9.5 • PGP9.5 • PGP95 • SPG79 • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) • ubiquitin carboxyl-terminal hydrolase isozyme L1 • ubiquitin thioesterase L1 • ubiquitin thiolesterase • Uch-L1
Product Information
Applications
Flow Cyt, ICC, IHC, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 50 and 100 of human Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) using the numbering given in entry NP_004172.2 (Gene ID 7345).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-216 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-216 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-216 · Lot 220429Open
Safety Data SheetPDF · A700-216Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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