Bethyl Laboratories
Rabbit anti-UNR Antibody Affinity Purified
Primary
Catalog #A303-158A
TargetUNR
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Upstream of N-ras (UNR) is also known as cold shock domain-containing E1 (CSDE1). UNR was identified as a mammalian transcription unit located immediately upstream of the N-ras gene. UNR contains 9 cold-shock domains and is a RNA-binding protein that is proposed to play a role in the control of protein translation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUNR
Antigen Species
Human
Gene ID7812
Gene Symbol
CSDE1
Gene Name
cold shock domain containing E1
Uniprot IDO75534
Protein Name
Cold shock domain-containing protein E1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UNR immobilized on solid support.
The epitope recognized by A303-158A maps to a region between residue 50 and 100 of human Upstream of NRAS using the numbering given in entry NP_001007554.1 (GeneID 7812).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-158A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-158A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-158AOpen
Citations / Publications
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