Bethyl Laboratories
Rabbit anti-USP1 Antibody Affinity Purified
Primary
Catalog #A301-698A
TargetUSP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 1 (USP1) has been found to play a role in the Fanconi anemia DNA repair pathway. USP1 specifically removes monoubiquitination from the Fanconi anemia D2 (FANCD2) protein and from the proliferating cell nuclear antigen (PCNA). Deubiquitination of FANCD2 and PCNA is critical to their role in the DNA damage response and DNA crosslink repair.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUSP1
Antigen Species
Human
Gene ID7398
Gene Symbol
USP1
Gene Name
ubiquitin specific peptidase 1
Uniprot IDO94782
Protein Name
Ubiquitin carboxyl-terminal hydrolase 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to USP1 immobilized on solid support.
The epitope recognized by A301-698A maps to a region between residue 300 and 350 of human ubiquitin-specific-processing protease 1 using the numbering given in entry NP_003359.3 (GeneID 7398).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-698A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-698A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-698AOpen
Citations / Publications
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