Bethyl Laboratories
Rabbit anti-USP19/ZMYND9 Antibody Affinity Purified
Primary
Catalog #A301-587A
TargetUSP19/ZMYND9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 19 (USP19) is a member of this family. USP19 is a 146 kDa protein that contains two CS domains and one MYND-type zinc finger.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 900 and 950
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUSP19/ZMYND9
Antigen Species
Human
Gene ID10869
Gene Symbol
USP19
Gene Name
ubiquitin specific peptidase 19
Uniprot IDO94966
Protein Name
Ubiquitin carboxyl-terminal hydrolase 19
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to USP19/ZMYND9 immobilized on solid support.
The epitope recognized by A301-587A maps to a region between residue 900 and 950 of human ubiquitin specific peptidase 19 (zinc finger MYND domain-containing protein 9) using the numbering given in entry NP_006668.1 (GeneID 10869).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-587A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-587A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-587AOpen
Citations / Publications
Services
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