Bethyl Laboratories
Rabbit anti-USP28 Antibody Affinity Purified
Primary
Catalog #A300-899A
TargetUSP28
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 28 (USP28) is a member of this family.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUSP28
Antigen Species
Human
Gene ID57646
Gene Symbol
USP28
Gene Name
ubiquitin specific peptidase 28
Uniprot IDQ96RU2
Protein Name
Ubiquitin carboxyl-terminal hydrolase 28
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to USP28 immobilized on solid support.
The epitope recognized by A300-899A maps to a region between residue 500 and 550 of human Ubiquitin Specific Peptidase 28 using the numbering given in entry NP_065937.1 (GeneID 57646).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-899A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-899A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-899AOpen
Citations / Publications
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