Bethyl Laboratories
Rabbit anti-USP37 Antibody Affinity Purified
Primary
Catalog #A300-927A
TargetUSP37
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 37 (USP37) is a member of this family.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 929 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUSP37
Antigen Species
Human
Gene ID57695
Gene Symbol
USP37
Gene Name
ubiquitin specific peptidase 37
Uniprot IDQ86T82
Protein Name
Ubiquitin carboxyl-terminal hydrolase 37
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to USP37 immobilized on solid support.
The epitope recognized by A300-927A maps to a region between residue 930 and the C-terminus (residue 979) of human Ubiquitin Specific Peptidase 37 using the numbering given in entry NP_065986.1 (GeneID 57695).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-927A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-927A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-927AOpen
Citations / Publications
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