Bethyl Laboratories
Rabbit anti-USP5/IsoT IHC Antibody Affinity Purified
Primary
Catalog #IHC-00313
TargetUSP5/IsoT
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetUSP5/IsoT
Antigen Species
Human
Gene ID8078
Gene Symbol
USP5
Gene Name
ubiquitin specific peptidase 5
Uniprot IDP45974
Protein Name
Ubiquitin carboxyl-terminal hydrolase 5
Alternate Names
deubiquitinating enzyme 5 • isopeptidase T • ISOT • testicular tissue protein Li 218 • ubiquitin carboxyl-terminal hydrolase 5 • ubiquitin isopeptidase T • ubiquitin specific protease 5 (isopeptidase T) • ubiquitin thioesterase 5 • ubiquitin thiolesterase 5 • ubiquitin-specific protease-5 (ubiquitin isopeptidase T) • ubiquitin-specific-processing protease 5
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to USP5/IsoT immobilized on solid support.
The epitope recognized by IHC-00313 maps to a region between residue 375 and 425 of human ubiquitin specific peptidase 5 (isopeptidase T) using the numbering given in entry CAA62690.1 (GeneID 8078).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00313Open
Product DatasheetPDF · IHC-00313 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00313 · Lot 1 (current lot)Open
Citations / Publications
Services
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