Bethyl Laboratories
Rabbit anti-UVRAG Antibody Affinity Purified
Primary
Catalog #A301-995A
TargetUVRAG
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 22 hours and 23 minutes for shipment by Tuesday, November 17
Product Description
UV radiation resistance associated gene (UVRAG) was first identified as a gene that could complement UV sensitivity in xeroderma pigmentosum cells. UVRAG may have tumor suppressor activity as mutations in the UVRAG gene have been associated with various human colon cancer cells and tissues. UVRAG has been shown to interact with Beclin 1 (Bcl-2-interacting protein), a protein that forms a complex with class III PI-3-kinase (PI3KC3) and is involved in autophagy. In this complex, UVRAG is proposed to function as a positive regulator of autophagy and a negative regulator of the growth of tumor cells.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUVRAG
Antigen Species
Human
Gene ID7405
Gene Symbol
UVRAG
Gene Name
UV radiation resistance associated
Uniprot IDQ9P2Y5
Protein Name
UV radiation resistance-associated gene protein
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UVRAG immobilized on solid support.
The epitope recognized by A301-995A maps to a region between residue 575 and 625 of human UV radiation resistance associated gene using the numbering given in entry NP_003360.2 (GeneID 7405).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-995A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-995A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-995AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1