Bethyl Laboratories
Rabbit anti-VAPB Antibody Affinity Purified
Primary
Catalog #A302-894A
TargetVAPB
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Vesicle-associated membrane protein-associated protein B/C (VAPB) is a type IV membrane protein found in plasma and intracellular vesicle membranes and may play a role in vesicle trafficking. Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8. ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Defects in VAPB are also a cause of spinal muscular atrophy autosomal dominant Finkel type (SMAF), also called late-onset spinal muscular atrophy Finkel type or spinal muscular atrophy proximal adult autosomal dominant [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O95292].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetVAPB
Antigen Species
Human
Gene ID9217
Gene Symbol
VAPB
Gene Name
VAMP associated protein B and C
Uniprot IDO95292
Protein Name
Vesicle-associated membrane protein-associated protein B/C
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to VAPB immobilized on solid support.
The epitope recognized by A302-894A maps to a region between residue 100 and 150 of human Vesicle-Associated Membrane Protein Associated Protein B using the numbering given in entry NP_004729.1 (GeneID 9217).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-894A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-894A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-894AOpen
Citations / Publications
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