Bethyl Laboratories
Rabbit anti-Varp Antibody Affinity Purified
Primary
Catalog #A302-997A
TargetVarp
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Varp (VPS9-ankyrin-repeat protein) is also known as ankyrin repeat domain-containing protein 27 (ANKRD27). Varp may function as a Rab21 guanine exchange factor and regulate endosome dynamics [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96NW4].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVarp
Antigen Species
Human
Gene ID84079
Gene Symbol
ANKRD27
Gene Name
ankyrin repeat domain 27
Uniprot IDQ96NW4
Protein Name
Ankyrin repeat domain-containing protein 27
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Varp immobilized on solid support.
The epitope recognized by A302-997A maps to a region between residue 225 and 275 of human Vps9 Domain and Ankyrin-Repeat-Containing Protein using the numbering given in entry NP_115515.2 (GeneID 84079).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-997A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-997A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-997AOpen
Citations / Publications
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