Bethyl Laboratories
Rabbit anti-Vinculin Antibody Affinity Purified
Primary
Catalog #A302-535A
TargetVinculin
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in vinculin are the cause of cardiomyopathy dilated type 1W, a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia [taken from NCBI Entrez Gene (GeneID: 7414)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1084 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVinculin
Antigen Species
Human
Gene ID7414
Gene Symbol
VCL
Gene Name
vinculin
Uniprot IDP18206
Protein Name
Vinculin
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Vinculin immobilized on solid support.
The epitope recognized by A302-535A maps to a region between residue 1016 and 1066 of human vinculin using the numbering given in entry NP_003364.1 (GeneID 7414).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A302-535A · Lot 3 (current lot)Open
Product DatasheetPDF · A302-535A · Lot 3 (current lot)Open
Safety Data SheetPDF · A302-535AOpen
Citations / Publications
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