Bethyl Laboratories
Rabbit anti-Vinculin IHC Antibody Affinity Purified
Primary
Catalog #IHC-00700
TargetVinculin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in vinculin are the cause of cardiomyopathy dilated type 1W, a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia [taken from NCBI Entrez Gene (GeneID: 7414)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1084 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetVinculin
Antigen Species
Human
Gene ID7414
Gene Symbol
VCL
Gene Name
vinculin
Uniprot IDP18206
Protein Name
Vinculin
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Vinculin immobilized on solid support.
The epitope recognized by IHC-00700 maps to a region between residue 1016 and 1066 of human vinculin using the numbering given in entry NP_003364.1 (GeneID 7414).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00700Open
Product DatasheetPDF · IHC-00700 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00700 · Lot 1 (current lot)Open
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