Bethyl Laboratories
Rabbit anti-Virilizer Antibody Affinity Purified
Primary
Catalog #A302-124A
TargetVirilizer
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1762 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVirilizer
Antigen Species
Human
Gene ID25962
Gene Symbol
VIRMA
Gene Name
KIAA1429
Uniprot IDQ69YN4
Protein Name
Protein virilizer homolog
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Virilizer immobilized on solid support.
The epitope recognized by A302-124A maps to a region between residue 1760 and 1810 of human Virilizer (KIAA1429) using the numbering given in entry NP_056311.2 (GeneID 25962).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A302-124A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-124A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-124AOpen
Citations / Publications
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