Bethyl Laboratories
Rabbit anti-Visfatin Antibody Affinity Purified
Primary
Catalog #A300-778A
TargetVisfatin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 2 hours and 49 minutes for shipment by Tuesday, November 10
Product Description
PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 441 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVisfatin
Antigen Species
Human
Gene ID10135
Gene Symbol
NAMPT
Gene Name
nicotinamide phosphoribosyltransferase
Uniprot IDP43490
Protein Name
Nicotinamide phosphoribosyltransferase
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Visfatin immobilized on solid support.
The epitope recognized by A300-778A maps to a region between residue 400 and 450 of human Visfatin (PBEF/NAMPT) using the numbering given in entry NP_005737.1 (GeneID 10135).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-778A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-778A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-778AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1