Rabbit anti-VMA21 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to VMA21 immobilized on solid support.

The epitope recognized by A305-701A maps to a region between residue 51 to 101 of human Vacuolar ATPase assembly integral membrane protein VMA21 using the numbering given in entry Q3ZAQ7.1 (GeneID 203547).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.