Bethyl Laboratories
Rabbit anti-Vps15 Antibody Affinity Purified
Primary
Catalog #A302-570A
TargetVps15
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
VPS (vacuolar protein sorting) proteins were originally characterized and studied in the yeast model and found to play a critical role in vesicle-mediated sorting of proteins to lysosome-like vacuoles. The human homolog of the yeast Vps15 protein was identified as a 150 kDa cellular protein that associates with the phosphatidylinositol (PtdIns) 3 kinase, Vps34p. Vps15 is a serine/threonine protein kinase that associates in a complex with beclin 1 and plays an important role in autophagy.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1075 and 1125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVps15
Antigen Species
Human
Gene ID30849
Gene Symbol
PIK3R4
Gene Name
phosphoinositide-3-kinase regulatory subunit 4
Uniprot IDQ99570
Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Vps15 immobilized on solid support.
The epitope recognized by A302-570A maps to a region between residue 1075 and 1125 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-570A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-570A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-570AOpen
Citations / Publications
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