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Rabbit anti-Vps15 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Vps15

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1308 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Country of Origin

USA

Additional Product Information

VPS (vacuolar protein sorting) proteins were originally characterized and studied in the yeast model and found to play a critical role in vesicle-mediated sorting of proteins to lysosome-like vacuoles. The human homolog of the yeast Vps15 protein was identified as a 150 kDa cellular protein that associates with the phosphatidylinositol (PtdIns) 3 kinase, Vps34p. Vps15 is a serine/threonine protein kinase that associates in a complex with beclin 1 and plays an important role in autophagy.

30849

PIK3R4

phosphoinositide-3-kinase regulatory subunit 4

Q99570

Phosphoinositide 3-kinase regulatory subunit 4

Alternate Names

p150; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide 3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.