/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-VTA1/LIP5 Antibody
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Primary Antibodies  

Rabbit anti-VTA1/LIP5 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

VTA1/LIP5

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 250 and 300

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

VTA1 is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VTA1 is thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. It is involved in the sorting and down-regulation of EGFR (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NP79].

51534

VTA1

vesicle trafficking 1

Q9NP79

Vacuolar protein sorting-associated protein VTA1 homolog

Alternate Names

C6orf55; dopamine-responsive gene 1 protein; DRG1; DRG-1; homolog of mouse SKD1-binding protein 1; HSPC228; LIP5; LYST-interacting protein 5; My012; SBP1; SKD1-binding protein 1; vacuolar protein sorting-associated protein VTA1 homolog; vesicle (multivesicular body) trafficking 1; Vps20-associated 1 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.