Bethyl Laboratories
Rabbit anti-WDHD1/AND-1 Antibody Affinity Purified
Primary
Catalog #A301-141A
TargetWDHD1/AND-1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
WDHD1/AND-1 contains multiple N-terminal WD domains and a C-terminal high mobility group (HMG) box. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. HMG boxes exhibit DNA binding activity and may be involved in chromatin assembly.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1079 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWDHD1/AND-1
Antigen Species
Human
Gene ID11169
Gene Symbol
WDHD1
Gene Name
WD repeat and HMG-box DNA binding protein 1
Uniprot IDO75717
Protein Name
WD repeat and HMG-box DNA-binding protein 1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDHD1/AND-1 immobilized on solid support.
The epitope recognized by A301-141A maps to a region between residue 1079 and 1129 of human WD repeat and HMG-box DNA binding protein 1 (acidic nucleoplasmic DNA-binding protein 1) using the numbering given in entry NP_009017.1 (GeneID 11169).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-141A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-141A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-141AOpen
Citations / Publications
Services
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