Bethyl Laboratories
Rabbit anti-WDR12 Antibody Affinity Purified
Primary
Catalog #A302-651A
TargetWDR12
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
WDR12 is a component of the PeBoW complex. PeBoW is required for the maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9GZL7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 373 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWDR12
Antigen Species
Human
Gene ID55759
Gene Symbol
WDR12
Gene Name
WD repeat domain 12
Uniprot IDQ9GZL7
Protein Name
Ribosome biogenesis protein WDR12
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR12 immobilized on solid support.
The epitope recognized by A302-651A maps to a region between residue 373 and 423 of human WD repeat domain 12 using the numbering given in entry NP_060726.3 (GeneID 55759).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-651A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-651A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-651AOpen
Citations / Publications
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