Bethyl Laboratories
Rabbit anti-WDR21A Antibody Affinity Purified
Primary
Catalog #A301-907A
TargetWDR21A
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
WDR21A (WD repeat-containing protein 21A) contains two WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. The function of WDR21A has not been characterized.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWDR21A
Antigen Species
Human
Gene ID26094
Gene Symbol
DCAF4
Gene Name
DDB1 and CUL4 associated factor 4
Uniprot IDQ8WV16
Protein Name
DDB1- and CUL4-associated factor 4
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR21A immobilized on solid support.
The epitope recognized by A301-907A maps to a region between residue 1 and 50 of human WD repeat-containing protein 21A using the numbering given in entry NP_056419.2 (GeneID 26094).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-907A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-907A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-907AOpen
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