Bethyl Laboratories
Rabbit anti-WDR4 Antibody Affinity Purified
Primary
Catalog #A301-554A
TargetWDR4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
Image 1 of 2
local_shipping
Order within 6 hours and 19 minutes for shipment by today
Product Description
WDR4 (WD repeat-containing protein 4) was identified in a search for candidate genes involved in the development of Down syndrome phenotypes. It is required for the 7-methylguanosine (m7G)-methyltransferase activity for tRNA. WDR bears two WD repeats. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWDR4
Antigen Species
Human
Gene ID10785
Gene Symbol
WDR4
Gene Name
WD repeat domain 4
Uniprot IDP57081
Protein Name
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR4 immobilized on solid support.
The epitope recognized by A301-554A maps to a region between residue 225 and 275 of human WD repeat domain 4 using the numbering given in entry NP_061139.2 (GeneID 10785).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-554A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-554A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-554AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1