Bethyl Laboratories
Rabbit anti-WDR43 Antibody Affinity Purified
Primary
Catalog #A302-478A
TargetWDR43
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 43 minutes for shipment by today
Product Description
WDR43 contains six WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. The function of WDR43 has not been characterized.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 627 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWDR43
Antigen Species
Human
Gene ID23160
Gene Symbol
WDR43
Gene Name
WD repeat domain 43
Uniprot IDQ15061
Protein Name
WD repeat-containing protein 43
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR43 immobilized on solid support.
The epitope recognized by A302-478A maps to a region between residue 627 and 677 of human WD repeat domain 43 using the numbering given in entry NP_055946.1 (GeneID 23160).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A302-478A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-478A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-478AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1