Bethyl Laboratories
Rabbit anti-WDR5 Antibody Affinity Purified
Primary
Catalog #A302-430A
TargetWDR5
HostRabbit
ClonalityPolyclonal
ReactivityRat, Mouse, Human
ApplicationIHC, WB
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Product Description
WD-repeat domain 5 (WDR5) is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WDR5 contains 7 WD repeats and is a core subunit of the human MLL and SET1 histone H3 lys4 methyltransferase complexes. WDR5 functions in facilitating the assembly of MLL and SET1 chromatin-modifiying complexes.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Rat, Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWDR5
Antigen Species
Human
Gene ID11091
Gene Symbol
WDR5
Gene Name
WD repeat domain 5
Uniprot IDP61964
Protein Name
WD repeat-containing protein 5
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR5 immobilized on solid support.
The epitope recognized by A302-430A maps to a region between residue 50 and 100 of human WD repeat domain 5 using the numbering given in entry NP_060058.1 (GeneID 11091).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-430A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-430A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-430AOpen
Citations / Publications
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